Fluorometric determination of lipids on thin-layer chromatogram sprayed with 2',7'-dichlorofluorescein.
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چکیده
منابع مشابه
Thin-layer Chromatography of Lipids
There has been a tendency to discount thin-layer chromatography (TLC) in recent years because of the availability of high-performance liquid chromatography (HPLC) methods that appeared to offer greater versatility and ease of quantification, amongst other reasons. However, TLC has made a comeback, largely because of the high reproducibility and lower costs of commercial pre-coated plates. It is...
متن کاملFluorometric determination of primary and secondary amines in blood and urine after thin-layer chromatography.
7-Chloro-4nitrobenzo -2oxa 1,3diazole reacts with primary and secondary amines to produce highly fluorescent compounds. The resulting emission spectra for primary and secondary amines are similar; however, when the excitation spectra of these compounds are determined when the emission wavelength is kept at 520 nm, distinctly different results are obtained. Thin.layer chromatography of suitably ...
متن کاملQuantitation of lipids by charring on thin-layer plates and scintillation quenching: application to ceramide determination.
A method is described for the quantitative determination of lipids after separation by thinlayer chromatography: the lipid spots are visualized with a charring spray and the darkness of the spots is determined by measuring the quenching produced when the powder is suspended in a scintillation counting gel containing a radioactive sample. A standard curve produced with 2 to 20 pg of ceramide was...
متن کاملarchitecture and engineering of nanoscale sculptured thin films and determination of their properties
چکیده ندارد.
15 صفحه اولDetermination of carotenes by thin-layer chromatography.
The carotenoids of yellow, red and tangerine tomato fruits were separated and identified by thin-layer chromatography (TLC). β-carotene is extracted with a mixture content hexan and acetone. The various systems of adsorbents and solvents for TLC of carote and tomato carotenes were examined, and it was revealed that the most suitable TLC system for separation and identification was silica gel pl...
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ژورنال
عنوان ژورنال: Agricultural and Biological Chemistry
سال: 1976
ISSN: 0002-1369,1881-1280
DOI: 10.1271/bbb1961.40.1253